Standard: API 32-30240
MUTAGENICITY EVALUATION STUDIES IN THE RAT BONE MARROW CYTOGENETIC ASSAY - IN THE MOUSE LYMPHOMA FORWARD MUTATION ASSAY - HYDRODESULFURIZED KEROSINE API SAMPLE 81-07
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Chromosome aberrations were determined in the bone marrow cells of Sprague-Dawley rats actively exposed intraperetoneally to API #81-07. Three dose levels were employed: 3.0 g/kg/day, 1.0 g/kg/day and 0.3 g/kg/day. Bone marrow was sampled 6, 24 and 48 hours after the acute dose. Five male and five female animals were exposed to each dose and negative ~ontrol level.
The structural aberration frequency in bone marrow cells of rats exposed to API #81-07 did not differ significantly from the negative control at any tested dose. The percentages of cells showing one or more structural aberrations or two or more structural aberrations were likewise similar to the negative controls, as was the frequency of numerical aberrations. These results were the same whether males or females or both sexes pooled were evaluated by the Student t-test. A concurrent positive control substance, triethylenemelamine, induced significant increases in structural aberrations in both the males and the females.
In vitro treatment of the mouse lymphoma L5178Y cell line did not induce Slgnificant increases in the mutant frequency at the thymidine kinase (TK) locus. The cells were exposed to API #81-07 for four hours both in the presence and absence of rat liver 59 metabolic activation. Treatments from 6.25 nl/ml to 37.5 nl/ml without activation and from 3.91 nl/ml to 62.5 nl/ml with S9 metabolic activation were assayed and a wide range of toxicities was induced without inducing significant increases in the mutant frequency. API #81-07 was therefore evaluated as inactive in the mouse lymphoma cell system.
|Organization:||American Petroleum Institute|
|Document Number:||api 32-30240|
|Change Type:||NEW ADDITION|
|Most Recent Revision:||YES|