CEN - EN 17299
Animal feeding stuffs: Methods of sampling and analysis - Screening and determination of authorized coccidiostats at additive and 1 % and 3 % cross-contamination level, and of non-registered coccidiostats and of one antibiotic at sub-additive levels, in compound feed with High Performance Liquid Chromatography - Tandem Mass Spectrometry detection (LC-MS/MS)
|Publication Date:||1 August 2019|
|ICS Code (Animal feeding stuffs):||65.120|
|ICS Code (Physicochemical methods of analysis):||71.040.50|
This document specifies a high performance liquid chromatographic - tandem mass spectrometry (HPLC-MS/MS) method for the simultaneous screening and/or determination of the eleven authorized coccidiostats (halofuginone hydrobromide, robenidine hydrochloride, nicarbazin, diclazuril, decoquinate, monensin sodium, salinomycin sodium, narasin, lasalocid A sodium, semduramicin sodium and maduramicin ammonium alpha) contents in compound feeds and feed materials of plant origin at additive and cross-contamination levels and of the five non-registered coccidiostats (ethopabate, clopidol, ronidazole, dimetridazole and amprolium) at sub-additive levels and for the screening of the prohibited furazolidone antibiotic at sub-additive level, in the same matrices. It has been fully validated only for poultry, cattle and pig compound feeds.
The range of application of the method is fit for the purpose of the screening and determination of all eleven registered coccidiostats at the values set by European legislation, of the non-registered coccidiostats and of the screening of the banned antibiotic.