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ISO 9308-1

Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora

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Organization: ISO
Publication Date: 15 September 2014
Status: active
Page Count: 18
ICS Code (Microbiology of water): 07.100.20
scope:

This part of ISO 9308 specifies a method for the enumeration of Escherichia coli (E. coli) and coliform bacteria. The method is based on membrane filtration, subsequent culture on a chromogenic coliform agar medium, and calculation of the number of target organisms in the sample. Due to the low selectivity of the differential agar medium, background growth can interfere with the reliable enumeration of E. coli and coliform bacteria, for example, in surface waters or shallow well waters. This method is not suitable for these types of water.

This part of ISO 9308 is especially suitable for waters with low bacterial numbers that will cause less than 100 total colonies on chromogenic coliform agar (CCA). These may be drinking water, disinfected pool water, or finished water from drinking water treatment plants.

Some strains of E. coli which are β-D-glucuronidase negative, such as Escherichia coli O157, will not be detected as E. coli. As they are β-D-galactosidase positive, they will appear as coliform bacteria on this chromogenic agar.

Document History

December 15, 2016
Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora AMENDMENT 1
A description is not available for this item.
ISO 9308-1
September 15, 2014
Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora
This part of ISO 9308 specifies a method for the enumeration of Escherichia coli (E. coli) and coliform bacteria. The method is based on membrane filtration, subsequent culture on a chromogenic...
June 15, 2007
Water quality — Detection and enumeration of Escherichia coli and coliform bacteria — Part 1: Membrane filtration method TECHNICAL CORRIGENDUM 1
A description is not available for this item.
September 15, 2000
Water Quality - Detection and Enumeration of Escherichia Coli and Coliform Bacteria - Part 1: Membrane Filtration Method
This part of ISO 9308 describes a reference method (Standard Test) for the detection and enumeration of Escherichia coli and coliform bacteria in water for human consumption. The Standard Test is...
January 1, 1990
Water Quality - Detection and Enumeration of Coliform Organisms, Thermotolerant Coliform Organisms and Presumptive Escherichia Coli - Part 1: Membrane Filtration Method
A description is not available for this item.

References

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