scope:

This TS specifies two horizontal methods for detection of the pathogenic bioserotypes of Y. enterocolitica and one for detection of Y. pseudotuberculosis by using real-time PCR-based methods. The methods detect the two pathogens by PCR and allow isolation of colonies. Y. pestis, the causative agent of bubonic and pneumonic plague harbour a variant of the ail gene as well and will be detected by the same primer/probe set as Y. pseudotuberculosis. However, Y. pestis is normally not associated with food. This TS is applicable to products for human consumption, animal feeding stuffs and environmental samples.