ISO TS 19006
Nanotechnologies - 5-(and 6)-Chloromethyl-2’,7’ Dichlorodihydrofluorescein diacetate (CMH2DCF- DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line
active, Most Current
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| Organization: | ISO |
| Publication Date: | 1 August 2016 |
| Status: | active |
| Page Count: | 28 |
| ICS Code (Physics. Chemistry): | 07.030 |
scope:
This document describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay.
The protocol in this document is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.
Document History
ISO TS 19006
August 1, 2016
Nanotechnologies - 5-(and 6)-Chloromethyl-2’,7’ Dichlorodihydrofluorescein diacetate (CMH2DCF- DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line
This document describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates...