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ISO 19040-3

Water quality - Determination of the estrogenic potential of water and waste water - Part 3: In vitro human cell-based reporter gene assay

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Organization: ISO
Publication Date: 1 August 2018
Status: active
Page Count: 48
ICS Code (Examination of biological properties of water): 13.060.70
scope:

This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay utilizing stably transfected human cells. This reporter gene assay is based on the activation of the human estrogen receptor alpha.

This method is applicable to:

- fresh water;

- waste water;

- aqueous extracts and leachates;

- eluates of sediments (fresh water);

- pore water;

- aqueous solutions of single substances or of chemical mixtures;

- drinking water;

- the limit of quantification (LOQ) of this method for the direct analysis of water samples is between 0,3 ng/l and 1 ng/l 17β-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper working range was evaluated [based on the results of the international interlaboratory trial (see Table F.3)] up to a level of 75 ng EEQ/l. Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre concentration of water samples can prove necessary if their estrogenic potential is below the given LOQ.

Document History

ISO 19040-3
August 1, 2018
Water quality - Determination of the estrogenic potential of water and waste water - Part 3: In vitro human cell-based reporter gene assay
This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay utilizing stably transfected human cells. This reporter...

References

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