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ASTM E2196

Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with Medium Shear and Continuous Flow Using Rotating Disk Reactor

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Organization: ASTM
Publication Date: 1 April 2017
Status: active
Page Count: 6
ICS Code (Microbiology in general): 07.100.01
scope:

This test method is used for growing a reproducible (1)2 Pseudomonas aeruginosa biofilm in a continuously stirred tank reactor (CSTR) under medium shear conditions. In addition, the test method describes how to sample and analyze biofilm for viable cells

Although this test method was created to mimic conditions within a toilet bowl, it can be adapted for the growth and characterization of varying species of biofilm (rotating disk reactor-repeatability and relevance (2))

This test method describes how to sample and analyze biofilm for viable cells. Biofilm population density is recorded as log10 colony forming units per surface area (rotating disk reactor-efficacy test method (3))

Basic microbiology training is required to perform this test method

The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use

This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee

Document History

ASTM E2196
April 1, 2017
Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with Medium Shear and Continuous Flow Using Rotating Disk Reactor
This test method is used for growing a reproducible (1)2 Pseudomonas aeruginosa biofilm in a continuously stirred tank reactor (CSTR) under medium shear conditions. In addition, the test method...
April 1, 2012
Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with Medium Shear and Continuous Flow Using Rotating Disk Reactor
This test method is used for growing a reproducible (1)2 Pseudomonas aeruginosa biofilm in a continuously stirred tank reactor (CSTR) under medium shear conditions. In addition, the test method...
April 1, 2007
Standard Test Method for Quantification of a Pseudomonas Aeruginosa Biofilm Grown with Shear and Continuous Flow Using a Rotating Disk Reactor
This test method is used for growing a repeatable2 Pseudomonas aeruginosa biofilm in a continuously stirred flow reactor. In addition, the test method describes how to sample and analyze biofilm for...
April 10, 2002
Standard Test Method for Quantification of a Pseudomonas Aeruginosa Biofilm Grown with Shear and Continuous Flow Using a Rotating Disk Reactor
This test method is used for growing a repeatable Pseudomonas aeruginosa biofilm in a continuously stirred flow reactor. In addition, the test method describes how to sample and analyze biofilm for...

References

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