Biotechnology - Cell counting - Part 1: General guidance on cell counting methods
|Publication Date:||1 January 2018|
|ICS Code (Biology. Botany. Zoology):||07.080|
This document defines terms related to cell counting for biotechnology. It describes counting of cells in suspension (generally cell concentration) and cells adhered to a substrate (generally area density of cells). It provides key considerations for general counting methods (including total and differential counting, and direct and indirect counting) as well as for method selection, measurement process, and data analysis and reporting.
This document is applicable to the counting of all cell types - mammalian and non-mammalian (e.g. bacteria, yeast) cells.
This document is not intended for counting of cells while in a tissue section or a biomaterial matrix.