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ISO 21446

Infant formula and adult nutritionals - Determination of trans and total (cis + trans) vitamin K1 content - Normal phase HPLC

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Organization: ISO
Publication Date: 1 January 2019
Status: active
Page Count: 26
ICS Code (General methods of tests and analysis for food products): 67.050
scope:

This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 μg/l to 80 μg/l trans vitamin K1, and the limit of quantification (LOQ) was estimated to be 0,4 μg/l for standards and 0,09 μg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.

Document History

ISO 21446
January 1, 2019
Infant formula and adult nutritionals - Determination of trans and total (cis + trans) vitamin K1 content - Normal phase HPLC
This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance...

References

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