scope:

This International Standard specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals, nuts and derived products. The limit of quantification for aflatoxin B 1 , and for the sum of aflatoxins B 1 , B 2 , G 1 and G 2 , is 8 µg/kg. The method has been validated for maize containing 24,5 µg/kg, for peanut butter containing 8,4 µg/kg, and for raw peanuts containing 16 µg/kg of total aflatoxins. It has also been shown that this method can be used for oilseed products, dried fruits and derived products.