scope:

This Technical Specification (CEN/TS 15606:2009) specifies a high performance liquid chromatographic (HPLC) method with UV-detection for the determination of acesulfame-K, aspartame, neohesperidinedihydrochalcone and saccharin in foodstuffs. The method has been fully validated [1] for the dialysis procedure through collaborative trial (see 8.2, 8.3 and Annex C), according to the IUPAC Harmonised Protocol [2], on the following analyte matrix combinations: - acesulfame-K (from 86 mg/l to 331 mg/l) and aspartame (from 97 mg/kg to 610 mg/l)in water-based drink, fruit-based drink, cheesecake with biscuit base, canned soup and instant chocolate drink - saccharin (from 70 mg/l to 97 mg/kg) in water-based drink, fruit-based drink, cheesecake with biscuit base and canned soup; - neohesperidine-dihydrochalcone (from 27 mg/l to 57 mg/kg)in water-based drink, fruit-based drink and canned soup.