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This document specifies a method for the determination of the toxic values of a wood preservative against larvae of Hylotrupes bajulus (Linnaeus), introduced into wood treated previously by full impregnation. This method is applicable to : - water-insoluble chemicals which are being studied as active insecticides or, -organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates or, -organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates or, - water-soluble materials, for example salts. The method is applicable wheth er or not the test specimens have been subjected to appropriate ageing procedures. This document supersedes EN 47:1988. Significant technical differences between this document and EN 47:1988 are as follows: a) introduction of new harmonised specifications for the test specimens used in the diverse biological tests; b) acknowledgement of the terms given in EN 1001-1; c) introduction of an informative Annex to take account of consideration for minimisation of environmental and health hazards caused by the use of this biological test.