scope:

Specifies a method for the determination of the composition of fatty acids which are in the 2-position of the triglyceride molecules in animal and vegetable fats and oils. Because of the nature of pancreatic lipase action ,the method is applicable only to fats and oils with a melting point below 45°C. The method is not unreservedly applicable to all fats and oils, particularly those containing substantial amounts of - fatty acids with 12 or fewer carbon atoms (copra oil, palm kernel oil, butyric butter fats);- fatty acids with 20 and more carbon atoms and of a high degree of unsaturation (more than four double bonds) (fish oil and marine animal oil);- fatty acids which have secondary groups containing oxygen.