scope:

This International Standard specifies a method for the isolation and enumeration of Pseudomonas aeruginosa in bottled water samples by a membrane filtration technique. This method can also be applied to other types of water with a low background flora, for example pool waters and waters intended for human consumption. Pseudomonas aeruginosa is an opportunistic pathogen of man that is capable of growth in water at very low nutrient concentrations. At source and during marketing, a natural mineral water or a spring water is to be free from Pseudomonas aeruginosa in any 250 ml sample examined (see, e.g. Council Directive 80/777/EEC[1] and Council Directive 96/70/EC[2]). Other bottled waters offered for sale are also to be free of Pseudomonas aeruginosa in any 250 ml sample (see, e.g. Council Directive 98/83/EC[3]). Other waters, including pool waters and water for human consumption, may sometimes be tested for Pseudomonas aeruginosa for reasons of public health. In these cases, it is typical to examine 100 ml volumes.