ISO FDIS 21115
Water quality - Determination of acute toxicity of water samples and chemicals to a fish gill cell-line (RTgill-W1)
|Publication Date:||28 December 2018|
|ICS Code (Examination of biological properties of water):||13.060.70|
This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24- well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).