ISO FDIS 21115

Water quality - Determination of acute toxicity of water samples and chemicals to a fish gill cell-line (RTgill-W1)

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Organization: ISO
Publication Date: 28 December 2018
Status: active
Page Count: 46
ICS Code (Examination of biological properties of water): 13.060.70
scope:

This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24- well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).

Document History

ISO FDIS 21115
December 28, 2018
Water quality - Determination of acute toxicity of water samples and chemicals to a fish gill cell-line (RTgill-W1)
This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in...

References

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