Method for Antifungal Disk Diffusion Susceptibility Testing of Nondermatophyte Filamentous Fungi; Approved Guideline
|Publication Date:||1 May 2010|
With a need to make antifungal susceptibility testing more readily available to the clinical laboratory, this CLSI document provides an established method for disk diffusion testing of moulds, zone interpretive criteria, and recommended control ranges for amphotericin B, caspofungin, itraconazole, posaconazole, and voriconazole.
The method described in this document is intended for testing moulds that cause invasive disease (Alternaria spp., Aspergillus spp., Bipolaris spp., Fusarium spp., Paecilomyces spp., Rhizopus oryzae [R. arrhizus] and other mucoraceous [zygomycetes] mould species, the Pseudallescheria boydii species complex, and Scedosporium prolificans).4 This method does not currently encompass the yeast or mould form of endemic dimorphic fungi or the dermatophytes.
The method described herein must be followed exactly to obtain reproducible results. When new problems are recognized or improvements in these criteria are developed, changes will be incorporated into future editions of this guideline and also distributed in periodic informational supplements.
This guideline is intended for use by, but not limited to, health care, academic, government, industry, or independent research organizations that perform antifungal susceptibility testing of filamentous fungi.