scope:

This International Standard specifies a method for the determination of the inhibition of growth of the macroalgae Ceramium tenuicorne by substances and mixtures contained in sea water or by waste water with salinities between 4 S and 32 S. This method is applicable for substances that are easily soluble in water. When toxicity is to be compared with other chemicals or waste waters it is recommended to perform the tests in artificial sea water. If the purpose of the testing is to assess and to predict effects in a specific receiving water body the tests should be conducted with algae adapted to the salinity in the receiving water body. In this case natural sea water from an uncontaminated site of the same properties should be used. NOTE With modifications as described in ISO 14442 and ISO 5667-16, the inhibitory effects of poorly soluble organic and inorganic materials, volatile compounds, metals, waste water, marine water samples and elutriates of sediments can be tested.