scope:

Part I of this standard describes the determination of hemoglobin concentration in human blood by the HiCN (cyanmethemoglobin) method. Accurate determination of hemoglobin concentration is:

• required for whole blood calibration procedures of automated hematology analyzers;

• necessary for the assignment of values to control materials used in hemoglobin measurement procedures;

• necessary in the evaluation of instruments and alternative methods for the measurement of hemoglobin concentration;

• applicable when patient red cell measurement means are used for calibration or control of hematology analyzers^10,11; and

• applicable in the routine hematology laboratory for diagnostic purposes and for monitoring the progress of therapy.

Thus, this standard is intended for all clinical laboratory personnel and for manufacturers of instruments, reagents, and calibration and control materials for the measurement of hemoglobin concentration.