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CLSI H42

Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Guideline

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Organization: CLSI
Publication Date: 1 May 2007
Status: inactive
Page Count: 88
scope:

The scope of this document is to establish performance guidelines for the identification and enumeration of lymphocyte subpopulations and the enumeration of CD34+ hematopoietic progenitors using immunofluorescence-based flow cytometry (FCM).

The working group recognizes that other, so-called nontraditional methodologies exist or are in development for enumeration of CD4+ T-lymphocytes (e.g., systems using microcapillary sample delivery or nonfluorescent cell detection; see Appendix C). Some of the issues discussed in this document are common to the use of any method for CD44 T-cell enumeration (e.g., sample collection and transport, safety issues, data reporting, and interpretation). However, issues such as sample preparation, instrument calibration, and quality control differ significantly for nontraditional methodologies and are not discussed in this document.

Presently, there are no universally accepted standards for precision, accuracy, and interlaboratory comparability in lymphocyte enumeration by FCM, General consensus was reached on the basic International Society for Hematotherapy and Graft Engineering (ISHAGE)9 guidelines for CD34 analysis, and this forms the basis of the technique described herein. It is beyond the scope of this document to establish general performance criteria and reference intervals. Therefore, it is each laboratory's responsibility to establish instrument performance criteria and staining characteristics for its own specific reagents.

Document History

May 1, 2007
Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Guideline
The scope of this document is to establish performance guidelines for the identification and enumeration of lymphocyte subpopulations and the enumeration of CD34+ hematopoietic progenitors using...
CLSI H42
May 1, 2007
Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Guideline
The scope of this document is to establish performance guidelines for the identification and enumeration of lymphocyte subpopulations and the enumeration of CD34+ hematopoietic progenitors using...
January 1, 1992
Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes; Tentative Guideline
A description is not available for this item.

References

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