scope:

This standard specifies a procedure for determining the degree of aerobic degradation of fully formulated lubricants. The organic material in a fully formulated lubricant is exposed in a synthetic aqueous medium under laboratory conditions to an inoculum from activated sludge. Biodegradation resulting in mineralisation of the organic material can be determined by measuring released CO2 in a total organic carbon (TOC-) analyser. The above mentioned method applies to fully formulated lubricants which a) are water-soluble, non-water soluble or emulsifiable b) are not toxic and not inhibitory to the test microorganisms at the test concentration. The presence of inhibitory effects can be determined