scope:

This International Standard specifies a method for the determination of the individual glucosinolates content in rapeseeds and rapeseed meals using high-performance liquid chromatography with gradient elution. NOTE 1 - : This method does not determine glucosinolates which are substituted on the glucose molecule, but these compounds are of little importance in commercial rapeseed. NOTE 2 - : This method was tested on rapeseeds and rapeseed meals (Brassica rapa, Brassica napus, and Brassica juncea) but is applicable to other plant materials on condition that the occurring glucosinolates previously identified are described in this standard. On the contrary, the quantitative analysis of the concerned glucosinolate(s) will not be carried out. NOTE 3 - : The analysis of glucosinolates content in rapeseed could also be done using an isocratic elution mode. This requires some modifications of the method (internal, standard, HPLC column and HPLC buffers) which is described in the informative Annex D at the end of this document.