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CLSI H44

Methods for Reticulocyte Counting (Automated Blood Cell Counters, Flow Cytometry and Supravital Dyes); Approved Guideline

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Organization: CLSI
Publication Date: 1 February 2004
Status: inactive
Page Count: 52
scope:

This document outlines the essential factors that affect automated reticulocyte counting using flow cytometric principles. The designated Class C comparative method (see Section 4, Definitions) is the new methylene blue (NMB) staining of blood collected in ethylenediaminetetraacetic acid (EDTA) anticoagulant. Methods to ensure acceptable precision and trueness of the calibration and quality control of the automated reticulocyte counting methods are outlined. Procedures to develop appropriate reference ranges are also outlined. Methods used to express the relative and absolute maturation of reticulocytes are discussed, primarily in an effort to guide in the standardization of these additional clinically useful reticulocyte parameters.

Document History

CLSI H44
February 1, 2004
Methods for Reticulocyte Counting (Automated Blood Cell Counters, Flow Cytometry and Supravital Dyes); Approved Guideline
This document outlines the essential factors that affect automated reticulocyte counting using flow cytometric principles. The designated Class C comparative method (see Section 4, Definitions) is...

References

This document references:
CLSI H20 - Reference Leukocyte (WBC) Differential Count (Proportional) and Evaluation of Instrumental Methods; Approved Standard
Published by CLSI on January 1, 2007
These recommendations cover performance testing of leukocyte (WBC) differential counting. Only those WBC found in normal (nondiseased) individuals will be addressed. These cell types are: neutrophils...
This document references:
ISO 3534-1 - Statistics Vocabulary and symbols Part 1: General statistical terms and terms used in probability
Published by ISO on October 15, 2006
This part of ISO 3534 defines general statistical terms and terms used in probability which may be used in the drafting of other International Standards. In addition, it defines symbols for a limited...
This document is referenced by:
CLSI H52 - Red Blood Cell Diagnostic Testing Using Flow Cytometry; Approved Guideline
Published by CLSI on March 1, 2014
This document establishes performance guidelines for applying the science of flow cytometry (FCM) to RBC diagnostic testing. It provides guidelines for: • Specimen collection, handling, and storage •...
This document is referenced by:
ISO 20391-1 - Biotechnology - Cell counting - Part 1: General guidance on cell counting methods
Published by ISO on January 1, 2018
This document defines terms related to cell counting for biotechnology. It describes counting of cells in suspension (generally cell concentration) and cells adhered to a substrate (generally area...
This document is referenced by:
CLSI H26 - Validation, Verification, and Quality Assurance of Automated Hematology Analyzers; Approved Standard
Published by CLSI on June 1, 2010
This document covers portions of the life cycle of an automated multichannel hematology system and provides guidance for validation, verification, calibration, quality assurance (QA), and quality...
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