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CLSI H44

Methods for Reticulocyte Counting (Automated Blood Cell Counters, Flow Cytometry and Supravital Dyes); Approved Guideline

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Organization: CLSI
Publication Date: 1 February 2004
Status: inactive
Page Count: 52
scope:

This document outlines the essential factors that affect automated reticulocyte counting using flow cytometric principles. The designated Class C comparative method (see Section 4, Definitions) is the new methylene blue (NMB) staining of blood collected in ethylenediaminetetraacetic acid (EDTA) anticoagulant. Methods to ensure acceptable precision and trueness of the calibration and quality control of the automated reticulocyte counting methods are outlined. Procedures to develop appropriate reference ranges are also outlined. Methods used to express the relative and absolute maturation of reticulocytes are discussed, primarily in an effort to guide in the standardization of these additional clinically useful reticulocyte parameters.

Document History

CLSI H44
February 1, 2004
Methods for Reticulocyte Counting (Automated Blood Cell Counters, Flow Cytometry and Supravital Dyes); Approved Guideline
This document outlines the essential factors that affect automated reticulocyte counting using flow cytometric principles. The designated Class C comparative method (see Section 4, Definitions) is...

References

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