CEN - EN 17252
Foodstuffs - Determination of phomopsin A in lupin seeds and lupin derived products by HPLC-MS/MS
| Organization: | CEN |
| Publication Date: | 1 January 2020 |
| Status: | active |
| Page Count: | 22 |
| ICS Code (Cereals, pulses and derived products): | 67.060 |
scope:
This document specifies a procedure for the determination of phomopsin A in lupin seeds and lupinderived products based on liquid chromatography with tandem mass spectrometry (LC-MS/MS). Several phomopsins exist, i.e. phomopsin A, B, C and D, but the method only deals with the quantitative measurement of phomopsin A due to lack of commercially available analytical reference standards for the other phomopsins.
The method has been validated for phomopsin A in naturally contaminated lupin seeds, lupin flour and crisp bread at levels ranging from approximately 5 μg/kg to 60 μg/kg.
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