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ASTM International - ASTM E1881-12(2020)

Standard Guide for Cell Culture Analysis with SIMS

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Organization: ASTM International
Publication Date: 1 December 2020
Status: active
Page Count: 3
ICS Code (Medical microbiology): 07.100.10
significance And Use:

5.1 The presence of cell growth medium complicates a direct analysis of cells with SIMS. Attempts to wash out the nutrient medium results in the exposure of cells to unphysiological reagents that... View More

scope:

1.1 This guide provides the Secondary Ion Mass Spectrometry (SIMS) analyst with a cryogenic method for analyzing individual tissue culture cells growing in vitro. This guide is suitable for frozen-hydrated and frozen-freeze-dried sample types. Included are procedures for correlating optical, laser scanning confocal and secondary electron microscopies to complement SIMS analysis.

1.2 This guide is not suitable for cell cultures that do not attach to the substrate.

1.3 This guide is not suitable for any plastic embedded cell culture specimens.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Document History

ASTM E1881-12(2020)
December 1, 2020
Standard Guide for Cell Culture Analysis with SIMS
5.1 The presence of cell growth medium complicates a direct analysis of cells with SIMS. Attempts to wash out the nutrient medium results in the exposure of cells to unphysiological reagents that may...
November 1, 2012
Standard Guide for Cell Culture Analysis with SIMS
5.1 The presence of cell growth medium complicates a direct analysis of cells with SIMS. Attempts to wash out the nutrient medium results in the exposure of cells to unphysiological reagents that may...
November 1, 2006
Standard Guide for Cell Culture Analysis with SIMS
The presence of cell growth medium complicates a direct analysis of cells with SIMS. Attempts to wash out the nutrient medium results in the exposure of cells to unphysiological reagents that may...
May 10, 1997
Standard Guide for Cell Culture Analysis with SIMS
1.1 This guide provides the Secondary Ion Mass Spectrometry (SIMS) analyst with a cryogenic method for analyzing individual tissue culture cells growing in vitro. This guide is suitable for...
May 10, 1997
Standard Guide for Cell Culture Analysis with SIMS
1.1 This guide provides the Secondary Ion Mass Spectometry (SIMS) analyst with a cryogenic method for analyzing individual tissue culture cells growing into vitro. This guide is suitable for...
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