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This document specifies a test method by artificial perspiration solution aqueous extraction for the determination of the aqueous extractable content of the following preservative agents in leather by liquid chromatography: ? 2-(thiocyanomethylthio)-benzothiazole (TCMTB); ? 4-chloro-3-methylphenol (PCMC); ? 2-phenylphenol (OPP); ? 2-octylisothiazol-3(2H)-one (OIT); This method can also be used to determine breakdown products of these preservative agents, which protect leather from microbiological attack.