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ASTM International - ASTM E1193-20

Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests

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Organization: ASTM International
Publication Date: 1 December 2020
Status: active
Page Count: 21
ICS Code (Microbiology of water): 07.100.20
ICS Code (Environmental impact assessment): 13.020.30
significance And Use:

5.1 Protection of an aquatic species requires prevention of unacceptable effects on populations in natural habitats. Toxicity tests are conducted to provide data that may be used to predict what... View More

scope:

1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material (added to dilution water, but not to food) on Daphnia magna Straus, 1820, during continuous exposure throughout a life-cycle using the renewal or flow-through techniques. These procedures also should be useful for conducting life-cycle toxicity tests with other invertebrate species and cladocerans from the same genus (for example, Daphnia pulex), although modifications might be necessary.

1.2 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications, these procedures can be used to conduct tests on temperature, dissolved oxygen, pH, and on such materials as aqueous effluents (also see Guide E1192), leachates, oils, particulate matter, sediments, and surface waters. The technique, (renewal or flow-through), will be selected based on the chemical characteristics of the test material such as high oxygen demand, volatility, susceptibility to transformation (biologically or chemically), or sorption to glass.

1.3 Modification of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of standard test procedures. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information on new concepts and procedures for conducting life-cycle toxicity tests with D. magna. Appendix X3 provides modifications for conducting the chronic toxicity test method with D. pulex Leydig, 1860.

1.4 This guide is arranged as follows:

Section
Referenced Documents 2
Terminology 3
Summary of Guide 4
Significance and Use 5
Apparatus 6
   Facilities 6.1
   Construction Materials 6.2
   Test Chambers 6.3
   Cleaning 6.4
   Acceptability 6.5
Reagents 7
   Purity of Reagents 7.1
Hazards 8
Dilution Water 9
   Requirements 9.1
   Source 9.2
   Treatment 9.3
   Characterization 9.4
Test Material 10
   General 10.1
   Stock Solutions 10.2
   Test Concentrations(s) 10.3
Test Organisms 11
   Species 11.1
   Age 11.2
   Source 11.3
   Brood Stock 11.4
   Food 11.5
   Handling 11.6
   Harvesting Young 11.7
   Quality 11.8
Procedure 12
   Experimental Design 12.1
   Dissolved Oxygen 12.2
   Temperature 12.3
   Loading 12.4
   Selection of Test System 12.5
   Beginning the Test 12.6
   Care and Maintenance 12.7
   Feeding 12.8
   Duration 12.9
   Biological Data 12.10
   Other Measurements 12.11
Analytical Methodology 13
Acceptability of Test 14
Calculation of Results 15
Report 16
Keywords 17
Appendixes
   Appendix X1   Statistical Guidance
   Appendix X2   Food
   Appendix X3   Modifications for Conducting Chronic Life Cycle Analysis Tests with

Daphnia Pulex

1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 8.

1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Document History

ASTM E1193-20
December 1, 2020
Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests
1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material (added to dilution water, but not to food) on Daphnia magna Straus, 1820, during...
December 1, 2012
Daphnia magna
1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material (added to dilution water, but not to food) on Daphnia magna Straus, 1820, during...
April 1, 2004
Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests
1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material (added to dilution water, but not to food) on Daphnia magna Straus, 1820, during...
October 10, 1997
Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests
1.1 This guide describes procedures for obtaining laboratory data concerning the adverse effects of a test material (added to dilution water, but not to food) on Daphnia magna Straus, 1820, during...
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