NEN-EN 17521
Foodstuffs - Determination of Alternaria toxins in tomato, wheat and sunflower seeds by SPE clean-up and HPLC-MS/MS
| Organization: | NEN |
| Publication Date: | 1 June 2020 |
| Status: | inactive |
| Page Count: | 40 |
| ICS Code (General methods of tests and analysis for food products): | 67.050 |
scope:
This document specifies a procedure for the determination of five Alternaria toxins in wheat, tomato puree and sunflower seeds by high-performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS).
The method has been validated with both naturally contaminated and spiked samples of wheat, tomato puree and sunflower seeds.
Validation levels for altenuene (ALT) ranged from 2,18 μg/kg to 13,8 μg/kg.
Validation levels for alternariol (AOH) ranged from 1,82 μg/kg to 46,7 μg/kg.
Validation levels for alternariol monomethyl ether (AME) ranged from 1,29 μg/kg to 47,2 μg/kg.
Validation levels for tentoxin (TEN) ranged from 5,29 μg/kg to 218 μg/kg.
Validation levels for tenuazonic acid (TEA) ranged from 41,8 μg/kg to 1 618 μg/kg.
Limits of quantification of 1 μg/kg for ALT (except in wheat and sunflower seeds), AOH and AME; 5 μg/kg for TEN and 10 μg/kg for TEA or lower are achievable using this method.
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