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ASTM International - ASTM E1295-22

Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia

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Organization: ASTM International
Publication Date: 1 January 2022
Status: active
Page Count: 21
ICS Code (Microbiology of water): 07.100.20
significance And Use:

5.1 Ceriodaphnia was first used as a toxicity test organism by Mount and Norberg (2). Introduced for use in effluent and ambient water evaluations, Ceriodaphnia have also been a... View More

scope:

1.1 This guide describes procedures for obtaining data concerning the adverse effects of an effluent or a test material (added to dilution water, but not to food) on Ceriodaphnia dubia Richard 1894, during continuous exposure throughout a portion of the organism's life. These procedures should also be useful for conducting life cycle toxicity tests with other Cladocera (Guide E1193), although modifications will be necessary.

1.2 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures, that can be measured accurately at the necessary concentrations in water. With appropriate modifications these procedures can be used to conduct tests on temperature, dissolved oxygen, pH, dissolved ions, and on such materials as aqueous effluents (see also Guide E1192), leachates, oils, particulate matter, sediments (see also Guide E1706), and surface waters. Renewal tests might not be applicable to materials that have high oxygen demand, are highly volatile, are rapidly biologically or chemically transformed, or sorb to test chambers. If the concentration of dissolved oxygen falls below 4 mg/L or the concentration of test material decreases by more than 20 % in test solution(s) at any concentration between renewals, more frequent renewals might be necessary.

1.3 Other modifications of these procedures might be justified by special needs or circumstances. Results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparisons of results obtained using modified and unmodified versions of these procedures might provide useful information on new concepts and procedures for conducting three-brood toxicity tests with C. dubia.

1.4 This guide is arranged as follows:

Section
Referenced Documents 2
Terminology 3
Summary of Guide 4
Significance and Use 5
Apparatus 6
   Facilities 6.1
   Construction Materials 6.2
   Test Chambers 6.3
   Cleaning 6.4
Reagents and Materials 7
Hazards 8
Dilution Water 9
   Requirements 9.1
   Source 9.2
   Treatment 9.3
   Characterization 9.4
Test Material 10
   General 10.1
   Stock Solution 10.2
   Effluent 10.3
   Test Concentration(s) 10.4
   Collection 10.5
   Sample Containers 10.6
   Preservation 10.7
   Treatment 10.8
Test Organisms 11
   Species 11.1
   Age 11.2
   Source 11.3
   Brood Stock 11.4
   Food 11.5
   Handling 11.6
   Quality 11.7
Procedure 12
   Demonstration of Feasibility 12.1
   Experimental Design 12.2
   Dissolved Oxygen 12.3
   Temperature 12.4
   Preparing Test Solutions 12.5
   Conditioning Test Chambers 12.6
   Beginning a Test 12.7
   Renewing Test Solutions 12.8
   Duration of Test 12.9
   Biological Data 12.10
   Other Measurements 12.11
   Test Material 12.12
Analytical Methodology 13
Acceptability of Test 14
Calculation 15
Report 16
Appendixes
   Food Appendix X1
   Culture Techniques Appendix X2
   Test Chambers Appendix X3
   Statistical Guidance Appendix X4

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 8.

1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Document History

ASTM E1295-22
January 1, 2022
Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia
1.1 This guide describes procedures for obtaining data concerning the adverse effects of an effluent or a test material (added to dilution water, but not to food) on Ceriodaphnia dubia Richard 1894,...
March 1, 2013
Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia
1.1 This guide describes procedures for obtaining data concerning the adverse effects of an effluent or a test material (added to dilution water, but not to food) on Ceriodaphnia dubia Richard 1894,...
April 1, 2006
Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia
1.1 This guide describes procedures for obtaining data concerning the adverse effects of an effluent or a test material (added to dilution water, but not to food) on Ceriodaphnia dubiaRichard 1894,...
December 10, 2001
Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia
1.1 This guide describes procedures for obtaining data concerning the adverse effects of an effluent or a test material (added to dilution water, but not to food) on Ceriodaphnia dubiaRichard 1894,...
December 10, 2001
Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia
1.1 This guide describes procedures for obtaining data concerning the adverse effects of an effluent or a test material (added to dilution water, but not to food) on Ceriodaphnia dubiaRichard 1894,...
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