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This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter of cigarette mainstream smoke using gas chromatography/mass spectrometry (GC/ MS) with cyclohexane as extraction solvent. This method is specified using ISO 20778 smoking parameters. An alternative method for the determination of B[a]P is specified in ISO 23906-1 with a different clean-up using methanol solvent. WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all the safety problems associated with its use. It is the responsibility of the user of this document to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.