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This document describes a method for the determination of individual intact glucosinolates in rapeseed by high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS). Progoitrin, gluconapin, glucobrassicanapin, glucobrassicin, nasturtiin, neoglucobrassicin and 4-methoxyglucobrassicin are quantitatively determined. Other glucosinolates such as 4-hydroxyglucobrassicin, glucnapoliferin, glucoalysin, can only be qualitatively detected when analytical standards become commercially available. The method has been in-house validated for rapeseeds in the range 0.05-60 mmol/kg for individual glucosinolates.