scope:

This amendment shall serve to update the current standard EN ISO 21571 according to the current needs. All alterations are listed hereafter: Contents list All methods listed in Annexes A and B shall appear in the contents list to read: Annex A (informative) Methods for DNA extraction 7 A.1 Preparation of PCR-quality DNA using phenol/chloroform-based DNA extraction methods 7 A.1.1 Basic phenol/chloroform method 7 A.1.2 Phenol/chloroform method: Protocol for starter cultures of fermented sausages 10 A.1.3 Phenol/chloroform method: Protocol for starter cultures of yoghurt 14 A.1.4 Phenol/chloroform method: Protocol for yeast and/or filamentous fungi harvested 18 A.2 Preparation of PCR-quality DNA using polyvinyl-pyrrolidone (PVP)-based DNA extraction methods 22 A.2.1 Basis PVP method 22 A.3 Preparation of PCR-quality DNA using the CTAB-based DNA extraction methods 24 A.3.1 Basic CTAB method 24 A.4 Preparation of PCR quality DNA using the silica-based DNA extraction methods 28 A.4.1 Basic silica method 28 A.5 Preparation of PCR quality DNA using guanidinium-chloroform-based DNA extraction methods 31 A.5.1 Basic guanidine-chloroform method 31 A.5.2 Guanidine chloroform method: Protocol for soybean lecithin 34 Annex B (informative) Methods for the quantitation of the extracted DNA 43 B.1 Basic ultraviolet spectrometric method 43 B.2 Agarose gel electrophoresis and ethidium bromide staining method 45 B.3 Real time PCR method for quantitation of extracted DNA 49 Bibliography 50