5.1 TLC is an inexpensive and simple technique that could be used to complement other analytical techniques within a general analytical scheme related to forensic fiber examination.

5.2 Consider the forensic analysis of fiber colorants using TLC for single fiber comparisons only when the sample size is adequate (that is, enough colorant can be extracted for analysis) and it is not possible to discriminate between the fibers of interest using other techniques, such as comparison microscopy and MSP. Larger fibrous units (for example, thread or tuft) can be treated as an individual sample if determined to be homogeneous. Do not treat fibers that cannot be directly related to each other as a collective sample for the purposes of TLC.

5.3 The extraction procedures carried out prior to TLC analysis can provide useful information about dye classification. TLC can provide qualitative information about dye components. Similar colors made up of different dye components can be differentiated using this technique. The application of TLC may serve to discriminate between fibers or it may support the possibility of fibers sharing a common source.

5.4 TLC can be prohibitively difficult or undesirable in some circumstances. Short lengths of fibers or pale-colored fibers can lack adequate amounts of colorant necessary to be examined by TLC. Dye extraction from some fibers can be impossible (2, 3). Some fiber types do not truly extract, but change or lose color. Reactive dyes are covalently bonded to the fiber and typically cannot be removed by conventional extraction methods, but can be released from cotton and wool by disrupting the fiber by enzymatic or chemical digestion, respectively (1). The desire to preserve evidence from deleterious change or for possible analysis by another examiner can preclude removing the color or employing a destructive method for analysis.