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ASTM International - ASTM E3011-22

Standard Practice for In vitro production of Clostridioides difficile Spores

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Organization: ASTM International
Publication Date: 1 October 2022
Status: active
Page Count: 3
ICS Code (Other standards related to microbiology): 07.100.99
significance And Use:

5.1 This practice describes a procedure for producing spore suspensions of C. difficile ATCC 700792, C. difficile ATCC 43598, or C. difficile ATCC 43599. The spore suspensions... View More

scope:

1.1 This practice is designed to propagate spores of Clostridioides difficile using liver broth.

1.2 It is the responsibility of the user of this practice to determine whether Good Laboratory Practices are required and follow when appropriate.

1.3 This practice should only be performed by those trained in microbiological techniques.

1.4 Units-The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Document History

ASTM E3011-22
October 1, 2022
Standard Practice for In vitro production of Clostridioides difficile Spores
1.1 This practice is designed to propagate spores of Clostridioides difficile using liver broth. 1.2 It is the responsibility of the user of this practice to determine whether Good Laboratory...
May 1, 2015
Standard Test Method for In vitro production of Clostridium Difficile Spores
1.1 This test method is designed to propagate spores of Clostridium difficile using liver broth. 1.2 It is the responsibility of the user of this test method to determine whether Good Laboratory...
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