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This method describes the procedures to be followed and precautions observed when performing reproducible measurements of the quantum efficiency of luminescent nanomaterials. Luminescent nanomaterials covered by this method include nano-objects such as quantum dots, nanophosphors, nanoparticles, nanofibers, nanocrystals, nanoplates, and structures containing these materials. The nanomaterials may be dispersed in either a liquid state (e.g., colloidal dispersion of quantum dots) or solid-state (e.g., nanofibers containing luminescent nanoparticles). This document covers both relative measurements of liquid state luminescent nanomaterials and absolute measurements of both solid and liquid state nanomaterials.