scope:

This International Standard specifies a test that uses the embryo-larval development of marine bivalves as a tool to assess the potential toxicity of chemicals or aqueous samples. It allows the determination of the concentration levels that result in an abnormality in embryo-larval development. This test is valid for salinity levels of between 20 and 40 for mussels and between 25 and 35 for oysters. This method applies to: . chemical substances and preparations; . marine and brackish waters; . streams and aqueous effluents (urban, agricultural, industrial effluents, etc.) as long as the salinity is adjusted and/or dilution is limited so that the aforementioned salinity ranges are respected; . aqueous extracts (pore water, elutriates, eluates, leachates, ...) from sediments and petroleum products.