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ASTM D4455

Standard Test Method for Enumeration of Aquatic Bacteria by Epifluorescence Microscopy Counting Procedure

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Organization: ASTM
Publication Date: 25 January 1985
Status: inactive
Page Count: 3
ICS Code (Microbiology of water): 07.100.20
scope:

This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridineorange epifluorescence direct-microscopic counting procedure. It is applicable to environmental waters.

Certain types of debris and other microorganisms may fluoresce in acridine orange-stained smears.

The test method requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications.2

Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends.

Approximately 104 cells/mL are required for detection by this test method.2

The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

This standard does not purport to address the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

2 The sole source of supply of the apparatus, Bacto Acridine Orange Stain, known to the committee at this time is Difco Laboratories, P.O. Box 1058, Detroit, MI 48201. If you are aware of alternative suppliers, please provide this information to ASTM International Headquarters. Your comments will receive careful consideration at a meeting of the responsible technical committee,1 which you may attend.

Document History

ASTM D4455
January 25, 1985
Standard Test Method for Enumeration of Aquatic Bacteria by Epifluorescence Microscopy Counting Procedure
This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridineorange epifluorescence direct-microscopic counting procedure. It is applicable to...
January 25, 1985
Standard Test Method for Enumeration of Aquatic Bacteria by Epifluorescence Microscopy Counting Procedure
This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridineorange epifluorescence direct-microscopic counting procedure. It is applicable to...
January 25, 1985
Standard Test Method for Enumeration of Aquatic Bacteria by Epifluorescence Microscopy Counting Procedure
1. Scope 1.1 This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridineorange epifluorescence direct-microscopic counting procedure. It is...
January 25, 1985
Standard Test Method for Enumeration of Aquatic Bacteria by Epifluorescence Microscopy Counting Procedure
This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. It is applicable to...

References

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