scope:

This International Standard describes a procedure for the indirect determination of 3-MCPD esters (bound 3- MCPD) and possible free 3-MCPD after alkaline catalysed ester cleavage and derivatisation with phenylboronic acid (PBA). Furthermore this standard enables the indirect determination of glycidyl esters (bound glycidol) under the assumption that no other substances are present that react at room temperature with inorganic chloride to generate 3-MCPD. The standard is applicable to solid and liquid fats and oils present as such or obtained by cautious extraction from processed fat and oil-containing foodstuffs. NOTE To extract fat from fat-containing foodstuffs, neither acidic nor alkaline cleavage procedures shall be used. Digestion procedures according to Weibull-Stoldt or similar shall not be used because significant amounts of 3-MCPD esters are formed via this route. Suitable solvents for fat extraction are methyl tertiary-butyl ether or mixtures of methyltertiary- butyl ether with hexane or petroleum ether. Under certain circumstances it is possible that 3-MCPD monoesters cannot be extracted completely with hexane or petroleum ether. Special techniques (e.g. fat extraction according to Röse- Gottlieb or Accelerated Solvent Extraction) have to be applied when samples like milk powder or infant formula might contain masked fat.