Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of yeasts and moulds - Part 2: Colony count technique in products with water activity less than or equal to 0,95
|Publication Date:||23 July 2008|
|ICS Code (Food microbiology):||07.100.30|
The present part of ISO 21527 standard described general guidance for the enumeration of viable osmophilic yeasts and xerophilic moulds in products intended for human consumption or feeding of animals, having a water activity £ 0.95 (dry fruits, cakes, jams, salted fish, grains, flours, nuts, spices, etc. [Annex A]), by means of the colony and propagule count technique at 25 ºC.
This method should not be applied to dehydrated products with water activity £ 0.60 (dehydrated cereals, oleaginous, spices, leguminous plants, seeds, powders for instant drinks, products dry for domestic animals, etc.) and does not allow the enumeration of spores of moulds.
The description of the mycologic flora is complementary research of the mycotoxins which is not the subject of this standard.
NOTE Owing to the nature of yeasts and moulds, the enumeration is subject to certain imprecision. For example, this method should not be used to enumerate halophilic xerophilic fungi (i.e. Polypaecilum pisce, Basipetospora halophila) as find in Indonesian dried fish.