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ASTM International - ASTM D4454-85(2002)

Standard Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy

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Organization: ASTM International
Publication Date: 25 January 1985
Status: inactive
Page Count: 3
ICS Code (Microbiology of water): 07.100.20
significance And Use:

Measurement of bacterial densities is generally the first step in establishing a relationship between bacteria and other biochemical processes.5 It is known that the classical plate... View More

scope:

1.1 This test method covers the detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. This test method is applicable to environmental waters and potable waters.

1.2 Certain types of debris and other microorganisms may fluoresce in acridine-orange stained smears.

1.3 The procedure described requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications.

1.4 Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends.

1.5 Approximately 104 cells/mL are required for detection by this test method.

1.6 Minimal cell size which allows the detection of formazan deposits is represented by bacteria of 0.4 m.2

1.7 This standard does not purport to address the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Document History

May 1, 2009
Standard Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy (Withdrawn 2015)
1.1 This test method covers the detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. This test method is applicable to...
December 10, 1997
Standard Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy
1.1 This test method covers the detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. This test method is applicable to...
ASTM D4454-85(2002)
January 25, 1985
Standard Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy
1.1 This test method covers the detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. This test method is applicable to...
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