ASTM International - ASTM D4455-85(2002)
Standard Test Method for Enumeration of Aquatic Bacteria by Epifluorescence Microscopy Counting Procedure
| Organization: | ASTM International |
| Publication Date: | 25 January 1985 |
| Status: | inactive |
| Page Count: | 3 |
| ICS Code (Microbiology of water): | 07.100.20 |
significance And Use:
Bacterial populations, as part of the microbial community in aquatic systems are actively involved in nutrient cycling. The significance of these populations is often difficult to ascertain... View More
scope:
1.1 This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. It is applicable to environmental waters.
1.2 Certain types of debris and other microorganisms may fluoresce in acridine orange-stained smears.
1.3 The test method requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications.
1.4 Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends.
1.5 Approximately 1014 cells/mL are required for detection by this test method.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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