ASTM International - ASTM E2317-04
Standard Guide for Conducting Renewal Microplate-Based Life-Cycle Toxicity Tests with a Marine Meiobenthic Copepod
|Publication Date:||1 April 2004|
|ICS Code (Other standards related to microbiology):||07.100.99|
1.1 This guide describes procedures for obtaining laboratory data concerning the adverse effects of a test material added to seawater, but not to food, on the marine copepod Amphiascus tenuiremis, during continuous exposures of individuals, from immediately after birth, until after the beginning of reproduction using a 200 μL renewal microplate-culturing
1.2 These procedures are applicable to most chemicals, either individually, or in formulations, commercial products, or known mixtures, that can be measured accurately at the necessary concentration in water. With appropriate modifications these procedures can be used to conduct tests on temperature, dissolved oxygen, and pH and on such materials as aqueous effluents (see also Guide E 1192), sediment pore waters, and surface waters. Renewal microplate tests might not be applicable to materials that have a high oxygen demand, are highly volatile, are rapidly transformed (biologically or chemically) in aqueous solutions, or are removed from test solutions in substantial quantities by the test chambers or organisms during the test. If the concentration of dissolved oxygen falls below 50 % of saturation, or the concentration of test material in the test solution decreases by more than 20 % between renewals, it might be desirable to renew the solutions more often.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory requirements prior to use.