ASTM International - ASTM E1493-06
Standard Guide for Identification of Bacteriophage M13 or Its DNA (Withdrawn 2014)
|Publication Date:||1 November 2006|
|ICS Code (Microbiology in general):||07.100.01|
significance And Use:
This guide is intended for use in a biotechnology laboratory when the need arises to identify a preparation containing M13 bacteriophage or DNA.
1.1 This guide covers the identification of bacteriophage M13 used in biotechnology.
1.2 There are many variants of M13 that have been developed specifically for cloning technology. These variants have foreign DNA inserted into the M13 genome, causing the M13 to differ in size and genotype.
1.3 If the M13 is to be used to construct a recombinant molecule, then the criteria described in Section 6 should be used to characterize the newly made DNA.