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# ASTM International - ASTM E1533-00(2006)

## Standard Practice for Indirect Detection of Mycoplasma in Cell Culture by 4-6-Diamidino-2-2 Phenylindole (DAPI) Staining (Withdrawn 2014)

inactive
 Organization: ASTM International Publication Date: 1 November 2006 Status: inactive Page Count: 3 ICS Code (Medical microbiology): 07.100.10
##### significance And Use:

Mycoplasma contamination of cell cultures is a common problem that can affect the growth, metabolism, and function of cultured animal cells. The ability to detect mycoplasma in cell cultures... View More

##### scope:

1.1 This practice covers procedures used for the detection of mycoplasma contamination by indirect DNA staining.

1.2 This practice does not cover direct methods for the detection of mycoplasma or other indirect methods such as enzymatical detection or DNA probes.

1.3 This practice does not cover methods for the identification of mycoplasma organisms.

1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

### Document History

ASTM E1533-00(2006)
November 1, 2006
Standard Practice for Indirect Detection of Mycoplasma in Cell Culture by 4-6-Diamidino-2-2 Phenylindole (DAPI) Staining (Withdrawn 2014)
Mycoplasma contamination of cell cultures is a common problem that can affect the growth, metabolism, and function of cultured animal cells. The ability to detect mycoplasma in cell cultures provides...
May 10, 2000
Standard Practice for Indirect Detection of Mycoplasma in Cell Culture by 4`-6-Diamidino-2-2 Phenylindole (DAPI) Staining
1.1 This practice covers procedures used for the detection of mycoplasma contamination by indirect DNA staining. 1.2 This practice does not cover direct methods for the detection of mycoplasma or...
May 10, 2000
Standard Practice for Indirect Detection of Mycoplasma in Cell Culture by 4'-6-Diamidino-2-2 Phenylindole (DAPI) Staining
1.1 This practice covers procedures used for the detection of mycoplasma contamination by indirect DNA staining. 1.2 This practice does not cover direct methods for the detection of mycoplasma or...