ASTM International - ASTM E578-07
Standard Test Method for Linearity of Fluorescence Measuring Systems
|Publication Date:||1 March 2007|
|ICS Code (Optical measuring instruments):||17.180.30|
significance And Use:
The range of concentration of a fluorescing substance in solution over which the fluorescence varies linearly with the concentration is the range most useful for quantitative analysis. This range... View More
The range of concentration of a fluorescing substance in solution over which the fluorescence varies linearly with the concentration is the range most useful for quantitative analysis. This range is affected by properties of the solution under analysis and by features of the measuring system. This test method provides a means of testing the performance of a fluorescence measuring system and of determining the concentration range over which the system is suitable for making a given quantitative analysis.
This test method is not meant for comparing the performance of different fluorescence measuring instruments.View Less
1.1 This test method covers a procedure for evaluating the limits of the linearity of response with fluorescence intensity of fluorescence-measuri
1.2 This test method has been applied to fluorescence-measuri
1.3 This test method has been applied to fluorescence-measuri
1.4 This test method is applicable to 10-mm pathlength cuvette formats and instruments covering a wavelength range within 190 to 900 nm. The use of other sample formats has not been established with this test method.
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.