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ASTM International - ASTM E2196-07

Standard Test Method for Quantification of a Pseudomonas aeruginosa Biofilm Grown with Shear and Continuous Flow Using a Rotating Disk Reactor

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Organization: ASTM International
Publication Date: 1 April 2007
Status: inactive
Page Count: 5
ICS Code (Microbiology in general): 07.100.01
significance And Use:

Bacteria that exist in a biofilm are phenotypically different from suspended cells of the same genotype. The study of biofilm in the laboratory requires protocols that account for this difference.... View More

scope:

1.1 This test method is used for growing a repeatable Pseudomonas aeruginosa biofilm in a continuously stirred flow reactor. In addition, the test method describes how to sample and analyze biofilm for viable cells.

1.2 In this test method, biofilm population density is recorded as log colony forming units per surface area.

1.3 Basic microbiology training is required to perform this test method. This standard does not claim to address all of the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety practices and determine the applicability of regulatory limitations prior to use.

Document History

April 1, 2017
Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with Medium Shear and Continuous Flow Using Rotating Disk Reactor
1.1 This test method is used for growing a reproducible (1)2 Pseudomonas aeruginosa biofilm in a continuously stirred tank reactor (CSTR) under medium shear conditions. In addition, the test method...
April 1, 2012
Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with Medium Shear and Continuous Flow Using Rotating Disk Reactor
1.1 This test method is used for growing a reproducible (1) Pseudomonas aeruginosa biofilm in a continuously stirred tank reactor (CSTR) under medium shear conditions. In addition, the test method...
ASTM E2196-07
April 1, 2007
Standard Test Method for Quantification of a Pseudomonas aeruginosa Biofilm Grown with Shear and Continuous Flow Using a Rotating Disk Reactor
1.1 This test method is used for growing a repeatable Pseudomonas aeruginosa biofilm in a continuously stirred flow reactor. In addition, the test method describes how to sample and analyze biofilm...
April 10, 2002
Standard Test Method for Quantification of a Pseudomonas aeruginosa Biofilm Grown with Shear and Continuous Flow Using a Rotating Disk Reactor
1.1 This test method is used for growing a repeatable Pseudomonas aeruginosa biofilm in a continuously stirred flow reactor. In addition, the test method describes how to sample and analyze biofilm...
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