ASTM International - ASTM E2196-07
Standard Test Method for Quantification of a Pseudomonas aeruginosa Biofilm Grown with Shear and Continuous Flow Using a Rotating Disk Reactor
|Publication Date:||1 April 2007|
|ICS Code (Microbiology in general):||07.100.01|
significance And Use:
Bacteria that exist in a biofilm are phenotypically different from suspended cells of the same genotype. The study of biofilm in the laboratory requires protocols that account for this difference.... View More
Bacteria that exist in a biofilm are phenotypically different from suspended cells of the same genotype. The study of biofilm in the laboratory requires protocols that account for this difference. Laboratory biofilms are engineered in growth reactors designed to produce a specific biofilm type. Altering system parameters will correspondingly result in a change in the biofilm. The purpose of this method is to direct a user in the laboratory study of biofilms by clearly defining each system parameter. This method will enable a person to grow, sample, and analyze a laboratory biofilm.View Less
1.1 This test method is used for growing a repeatable Pseudomonas aeruginosa biofilm in a continuously stirred flow reactor. In addition, the test method describes how to sample and analyze biofilm for viable cells.
1.2 In this test method, biofilm population density is recorded as log colony forming units per surface area.
1.3 Basic microbiology training is required to perform this test method. This standard does not claim to address all of the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety practices and determine the applicability of regulatory limitations prior to use.