scope:

1.1 This practice is used for the recovery of viruses from wastewater sludges and favors the enteroviruses.

1.2 Both procedures are applicable to raw, digested, and dewatered sludges.

Sections Procedure A---Adsorption 6 to 10 Procedure B---Sonication 11 to 15

1.3 This practice was tested on standardized sludges as described in 10.1 and 17.1. It is the user's responsibility to ensure the validity of this practice for untested matrices.

1.4 This standard may involve hazardous materials, operations, and equipment. This standard does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

1.5 Only adequately trained personnel should be allowed to perform these procedures and should use safety precautions recommended by the U.S. Public Health Service, Center for Disease Control, for work with potentially hazardous biological organisms.