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ASTM International - ASTM E1218-97a

Standard Guide for Conducting Static 96-h Toxicity Tests with Microalgae

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Organization: ASTM International
Publication Date: 10 October 1997
Status: inactive
Page Count: 14
ICS Code (Microbiology of water): 07.100.20
scope:

1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material added to growth medium on growth of certain species of freshwater and saltwater microalgae during a 96-h static exposure. These procedures will probably be useful for conducting short-term toxicity tests with other species of algae, although modifications might be necessary.

1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting toxicity tests with microalgae.

1.3 These procedures are applicable to many chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications, these procedures can be used to conduct tests on temperature, dissolved oxygen, and pH and on such materials as aqueous effluents (see also Guide E 1192), leachates, oils, particulate matter, sediments, and surface waters. Static tests might not be applicable to materials that have a high oxygen demand, are highly volatile, are rapidly biologically or chemically transformed in aqueous solutions, or are removed from test solutions in substantial quantities by the test vessels or organisms during the test. However, practical flow-through test procedures with microalgae have not been developed.

1.4 Results of tests using microalgae should usually be reported in terms of the 96-h IC50 (see ) based on reduction in growth. In some situations, it might only be necessary to determine whether a specific concentration unacceptably affects the growth of the test species or whether the IC50 is above or below a specific concentration.

1.5 This guide is arranged as follows:

Section
Referenced Documents2
Terminology3
Summary of Guide4
Significance and Use5
Hazards7
Apparatus6
Facilities6.1
Equipment6.2
Test Vessels6.3
Cleaning6.4
Acceptability6.5
Growth Medium8
Test Material9
General9.1
Stock Solution9.2
Test Concentration(s)9.3
Test Organisms10
Species10.1
Source10.2
Culture10.3
Quality10.4
Procedure11
Experimental Design11.1
Temperature11.2
Illumination11.3
Beginning the Test11.4
Gas Exchange11.5
Duration of Test11.6
Biological Data11.7
Other Measurements11.8
Determination of Algistatic and Algicidal Effects11.8.5
Analytical Methodology12
Acceptability of Test13
Calculation14
Report15
Keywords16

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 7.

Document History

November 1, 2021
Standard Guide for Conducting Static Toxicity Tests with Microalgae
1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material added to growth medium on growth of certain species of freshwater and saltwater...
December 1, 2012
Standard Guide for Conducting Static Toxicity Tests with Microalgae (Withdrawn 2021)
1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material added to growth medium on growth of certain species of freshwater and saltwater...
April 1, 2004
Standard Guide for Conducting Static Toxicity Tests with Microalgae
1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material added to growth medium on growth of certain species of freshwater and saltwater...
April 1, 2004
Standard Guide for Conducting Static Toxicity Tests with Microalgae
1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material added to growth medium on growth of certain species of freshwater and saltwater...
ASTM E1218-97a
October 10, 1997
Standard Guide for Conducting Static 96-h Toxicity Tests with Microalgae
1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material added to growth medium on growth of certain species of freshwater and saltwater...
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