This practice is not recommended for acceptance testing because the information obtained is qualitative rather than quantitative in nature.

The response of fibers to the dye mixture is indicative of the uniformity of dyeing, the probability of dyeing defects due to neps, and the difficulties to be expected from heterogeneity of fibers that differ in dyeing characteristics. In general, the red dye is retained predominantly by the thick-walled fibers and the green by the thin-walled fibers.

The color response of the specimens to the dyes in the binary mixture reflects the behavior to be expected with many other dyes. The colors from red to green may be influenced by factors such as weather during the growing period, exposure of open bolls in the field before harvest, natural color, fineness, and degree of wall development.