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ASTM International - ASTM E1286-00

Standard Guide for Identification of Herpes Simplex Virus or its DNA

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Organization: ASTM International
Publication Date: 10 September 2000
Status: inactive
Page Count: 3
ICS Code (Medical microbiology): 07.100.10
scope:

1.1 This guide covers laboratory characterization procedures sufficient to identify purified specimens of HSV types 1 and 2 (HSV-1 and HSV-2) or HSV-1 DNA and HSV-2 DNA used in biotechnology. For cases in which identification of HSV DNA specimens is required, the characterization criteria of 6.2 and 6.3 of this guide are sufficient.

1.2 This guide does not cover the identification of HSV in HSV-infected host cells. To apply this guide to such a case, it would first be necessary to isolate the virus from such samples using standard techniques of HSV purification. This guide does not cover characterization of segments of HSV DNA or of vectors containing HSV DNA segments.

1.3 This guide does not cover the specific methodology used in the identification characterization. It does not address the question of degree of purity required for herpesvirus preparations: this would vary depending on the particular biotechnology use of the virus.

1.4 Warning--Laboratory work involving herpes simplex viruses can be hazardous to personnel. Precaution: Biosafety 2 level facilities are recommended (1). Safety guidelines shall be adhered to according to NCCLS M29-T2 and other recommendations (1).

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Document History

November 1, 2006
Standard Guide for Identification of Herpes Simplex Virus or Its DNA (Withdrawn 2014)
1.1 This guide covers laboratory characterization procedures sufficient to identify purified specimens of HSV types 1 and 2 (HSV-1 and HSV-2) or HSV-1 DNA and HSV-2 DNA used in biotechnology. For...
ASTM E1286-00
September 10, 2000
Standard Guide for Identification of Herpes Simplex Virus or its DNA
1.1 This guide covers laboratory characterization procedures sufficient to identify purified specimens of HSV types 1 and 2 (HSV-1 and HSV-2) or HSV-1 DNA and HSV-2 DNA used in biotechnology. For...
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