ASTM E1326

Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria

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Organization: ASTM
Publication Date: 10 October 1998
Status: inactive
Page Count: 3
ICS Code (Medical microbiology): 07.100.10
scope:

1. Scope

1.1 The purpose of this guide is to assist users and producers of nonconventional tests in determining the applicability of the test for processing different types of samples and evaluating the accuracy of the results. Conventional procedures such as the Heterotrophic (Standard) Plate Count, the Most Probable Number (MPN) method and the Spread Plate are widely cited and accepted for the enumeration of microorganisms. However, these methods have their limitations, such as performance time and degree of accuracy. It is these limitations that have recently led to the marketing of a variety of non-conventional procedures, test kits and instruments.

1.2 A conventional test is one that is widely accepted and published as a standard microbiological method or related procedure. A new, nonconventional test method will attempt to provide the same information through the measurement of a different parameter. This guide is designed for comparing levels of bacteria recovered from samples by the Heterotrophic Plate Count Procedure to the equivalent units determined with a nonconventional test.

1.3 It is recognized that the Heterotrophic Plate Count does not recover all microorganisms present in a product or a system (1). When this problem occurs during the characterization of a microbiological population, alternate standard enumeration procedures may be necessary, as in the case of sulfate-reducing bacteria. At other times, chemical methods that measure the rates of appearance of metabolic derivatives or the utilization of contaminated product components might be indicated. In evaluating nonconventional tests, the use of these alternate standard procedures may be the only means available for establishing correlation. In such cases, this guide can serve as a reference for those considerations.

1.4 Since there are so many types of tests that could be considered nonconventional, it is impossible to recommend a specific test protocol with statistical analyses for evaluating the tests. Instead, this guide should assist in determining what types of tests should be considered to verify the utility and identify the limitations of the nonconventional test.

Document History

October 1, 2015
Standard Guide for Evaluating Non-culture Microbiological Tests
The purpose of this guide is to assist users and producers of non-culture microbiological tests in determining the applicability of the test for processing different types of samples and evaluating...
May 1, 2015
Standard Guide for Evaluating Non-culture Microbiological Tests Used for Enumerating Bacteria
The purpose of this guide is to assist users and producers of non-culture tests in determining the applicability of the test for processing different types of samples and evaluating the accuracy of...
October 1, 2013
Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
The purpose of this guide is to assist users and producers of nonconventional tests in determining the applicability of the test for processing different types of samples and evaluating the accuracy...
October 1, 2008
Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
The purpose of this guide is to assist users and producers of nonconventional tests in determining the applicability of the test for processing different types of samples and evaluating the accuracy...
November 1, 2006
Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
The purpose of this guide is to assist users and producers of nonconventional tests in determining the applicability of the test for processing different types of samples and evaluating the accuracy...
ASTM E1326
October 10, 1998
Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
1. Scope 1.1 The purpose of this guide is to assist users and producers of nonconventional tests in determining the applicability of the test for processing different types of samples and evaluating...
January 1, 1990
Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
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